Primerize Documentation¶

Primerize (previously named NA_thermo), is an archive of Python and MATLAB scripts for primer design and nucleic acid thermodynamic scripts developed by the Das Lab at Stanford University for high-throughput RNA synthesis and design.

The algorithm designs forward (sense strand) and reverse (anti-sense strand) primers that minimize the total length, and therefore the total synthesis cost, of the oligonucleotides. Although developed independently, Primerize is a special case of the general ‘Gapped Oligo Design’ algorithm, optimizing the mispriming score and sequence span instead of T_m.

An online user-friendly GUI is available as the Primerize Server.

The code for Primerize is available at <https://github.com/ribokit/Primerize>`_