PCR Assembly Primer Design
primerize.thermo
Module¶primerize.thermo.
Nearest_Neighbor_Parameter
¶Bases: primerize.thermo.Singleton
Wrapper object of Nearest Neighbor parameters; for internal use.
T
¶float
delH_NN
¶numpy.array(float(4, 4))
delS_NN
¶numpy.array(float(4, 4))
delG_NN
¶numpy.array(float(4, 4))
delH_AT_closing_penalty
¶numpy.array(float(1, 4))
delS_AT_closing_penalty
¶numpy.array(float(1, 4))
delG_AT_closing_penalty
¶numpy.array(float(1, 4))
delH_mismatch
¶numpy.array(float(4, 4, 4))
delS_mismatch
¶numpy.array(float(4, 4, 4))
delG_mismatch
¶numpy.array(float(4, 4, 4))
delH_init
¶float
del_init
¶float
Note
This class
follows the singleton pattern so that only one instance is created. An instance is already initialized as primerize.Nearest_Neighbor
.
primerize.thermo.
Singleton
¶Bases: object
Base class for singleton pattern
primerize.thermo.
calc_Tm
(sequence, DNA_conc=1e-05, monovalent_conc=1.0, divalent_conc=0.0)¶Calculate melting temperature for a given sequence
Parameters: |
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Returns: |
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Last updated on Jun 22, 2017.