primerize.primerize_custom Module¶

class primerize.primerize_custom.Primerize_Custom(*args, **kwargs)¶

Bases: Singleton

Construct a worker for Custom Primer Design (Custom Plates).

Parameters:

• offset – int: (Optional) Sequence numbering offset, which is one minus the final number of the first nucleotide.

• COL_SIZE – int: (Optional) Column width for assembly output. Positive number only.

• prefix – str: (Optional) Construct prefix/name.

Returns:

primerize.Primerize_Custom

Note

This class follows the singleton pattern so that only one instance is created. An instance is already initialized as primerize.Primerize_Custom.

design(sequence, primer_set=[], mut_list=[], offset=None, prefix=None, is_force=False)¶

Run design code to get customized plates for input sequence and specified list of constructs. Current worker parameters are used for nonspecified optional arguments.

Parameters:

• job_1d – primerize.Design_Single: Result of primerize.Primerize_1D.design(). Its sequence, primer_set, and prefix are used.

• mut_list – primerize.Construct_List: List of constructs for design.

• offset – int: (Optional) Sequence numbering offset.

Returns:

primerize.Design_Plate

get(key)¶

Get current worker parameters.

Parameters:

key – str: Keyword of parameter. Valid keywords are 'offset', 'COL_SIZE', 'prefix'; case insensitive.

Returns:

value of specified key.

Raises:

AttributeError – For illegal key.

reset()¶

Reset current worker parameters to default.

set(key, value)¶

Set current worker parameters.

Parameters:

• key – str: Keyword of parameter. Valid keywords are the same as get().

• value – (auto): New value for specified keyword. Type of value must match key.

Raises:

• AttributeError – For illegal key.

• ValueError – For illegal value.