primerize.primerize_custom Module¶

class primerize.primerize_custom.Primerize_Custom(offset=0, COL_SIZE=142, prefix='lib')¶

Bases: primerize.thermo.Singleton

Construct a worker for Custom Primer Design (Custom Plates).

Parameters:	offset -- int: (Optional) Sequence numbering offset, which is one minus the final number of the first nucleotide. COL_SIZE -- int: (Optional) Column width for assembly output. Positive number only. prefix -- str: (Optional) Construct prefix/name.
Returns:	primerize.Primerize_Custom

Note

This class follows the singleton pattern so that only one instance is created. An instance is already initialized as primerize.Primerize_Custom.

design(sequence, primer_set=[], mut_list=[], offset=None, prefix=None, is_force=False)¶

Run design code to get customized plates for input sequence and specified list of constructs. Current worker parameters are used for nonspecified optional arguments.

Parameters:	job_1d -- primerize.Design_Single: Result of primerize.Primerize_1D.design(). Its sequence, primer_set, and prefix are used. mut_list -- primerize.Construct_List: List of constructs for design. offset -- int: (Optional) Sequence numbering offset.
Returns:	primerize.Design_Plate

get(key)¶

Get current worker parameters.

Parameters:	key -- str: Keyword of parameter. Valid keywords are 'offset', 'COL_SIZE', 'prefix'; case insensitive.
Returns:	value of specified key.
Raises:	AttributeError -- For illegal key.

reset()¶

Reset current worker parameters to default.

set(key, value)¶

Set current worker parameters.

Parameters:	key -- str: Keyword of parameter. Valid keywords are the same as get(). value -- (auto): New value for specified keyword. Type of value must match key.
Raises:	AttributeError -- For illegal key. ValueError -- For illegal value.