RiboKit : HiTRACE

High-Throughput Robust Analysis for Capillary Electrophoresis

More Practice

We have prepared 12 datasets (1D and 2D) for more practice of manual Sequence Assignment (Step #4). They are previous data collected and annotated by experts from our lab. The expert assignments are included in the .mat workspace files.

To practice your skills, you need to do the sequence assignment on your own, and then compare your results to our ‘standarad’ answers. You may want to track down differences, and learn through this experience how to adjust them. This serves well for gaining experience on scenarios of common mistakes and how to fix them.

Instructions

load('practice_1D_more_1.mat', 'd_align', 'd_ref_align', 'ylimit');

This is because we did not include the raw CE trace files. Thus, the quick_look() step is skipped for these practices, and you should resume from Step #2.

The d_align variable holds the essential data from quick_look() (Step #1). The above command loads it, among d_ref_align and ylimit from the .mat file.

d_align = d_align_before_more_alignment;

This is because the expert .mat result already have gone through the entire script, overwrote d_align with d_align_dp_fine. For datasets that no additional alignment were performed (see corresponding .m scripts), ignore this.

Do not overwrite the downloaded .mat files.

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