Experimental Protocols
| Protocol | Description |
|---|---|
| Order Oligo | Order primers for PCR assembly |
| Make DNA | PCR (polymerase chain reaction) primer assembly of DNA tempaltes, and purification |
| Make RNA | IVT (in vitro transcription) of RNA, and purification |
| RNA QC | Check RNA Quality |
| Structure Mapping | High-Throughput RNA Chemical Mapping by Capillary Electrophoresis |
| MAP-Seq | RNA Chemical Mapping read out by Next-Generation Sequencing |
| MOHCA-Seq | Multiplexed Hydroxyl Radical (•OH) Cleavage Analyzed by Sequencing |
Built with Jekyll using a RiboKit Theme . Hosted on GitHub Pages.